Efficacy of Aloe vera variety Barbadensis on bacterial isolates from cultured freshwater fish in Kenya

Aloe vera variety barbadensis is a potential alternative antimicrobial plant extract that has been widely used in humans. The objective of this study was to investigate the susceptibility selected bacterial isolates from cultured freshwater fish different concentrations crude extract. Using Minimum inhibitory concentration method by Broth dilution technique, diluted distilled water 250 mg/ml, 125mg/ml, 62.5mg/ml, 31.25mg.ml, 15.63mg/ml,7.85 3.93 mg/ml and 1.96 mg/ml. Eighteen (18) namely Enterococcus faecalis NT2I3, Lysinibacillus fusiformis MT8G4, Bacillus cereus NC5K2, (MT7G3), Micrococcus luteus (MC3K2), Kurthia gibsoni (NT5G5), thuringiensis NT4G4, Aeromonas hydrophila NT5K2, MT7G2, Pseudomonas mendocina NT5G2, putida MT7I5, Enterobacter cloaca MT6G2, veronii NT4G1, Citrobacter braakii NC3S2, freundii NC3G3, Escherichia coli NC2G1(ii), MT2G1 Hafnia alvei MC3K1were each suspended physiological saline turbidity matching 0.5 Mc Farland opacity tube. Then for concentration, 1 ml mixed with 0.1 respective suspension incubated at 37 °C 24 hours. Only isolate (Kurthia gibsonii) susceptible MIC 62.5 vera. Seven (Bacillus MT7G3, luteus, veronii, NC2G1 (ii)) were 125 Five NC3K2, thuringiensis, putida, feacalis) 250mg/ml Resistance highest observed five fusiformis, mendocina, freundii, MT2G1. shown be efficacious against most Gram positive negative bacteria isolated between